According to the World Health Organization (WHO), antimicrobial resistance (AMR) kills about 700,000 people worldwide annually, with the majority of those deaths occurring in developing countries. To address this health crisis, Jordan’s Ministry of Health (MOH) is implementing a National Action Plan (NAP) to combat AMR. The US Agency for International Development (USAID) Medicines, Technologies, and Pharmaceutical Services (MTaPS) Program designed the rational use of antimicrobials (RUA) program to help the MOH with the implementation of the NAP. In this program, clinical and administrative teams at MOH hospitals developed key protocols to update and standardize the use of antimicrobials for treatment and prophylaxis in selected priority clinical areas.